Nucleic Acids & DNA markers

Description

Product Details

The 1kbp DNA Ladder is suitable for sizing double-stranded DNA from 1kb to 8kb. The 4kb band has increased intensity to serve as reference point. The double-stranded ladder can be visualized on 1% to 2% agarose gels after ethidium bromide staining. 

Preparation: Complete digestion of a plasmid mix with appropriate restriction enzyme yields the following 7 discrete fragments (in kilobase pairs): 8, 6, 5, 4, 3, 2, 1. 

Storage conditions:   

• 10 mM Tris-HCl (pH 8.0)  
• 1 mM EDTA 
• Store at -20ºC 

 Note: Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH₂O and subsequently heated.