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  Restriction endonucleases

Alu I

AluI is a restriction enzyme purified from Arthrobacter luteus (ATCC 21606)

Reagents supplied: 10x R1 and 10x EQ buffer

Unit definition: One unit is the amount of enzyme required to digest 1 μg of Lambda DNA in 60 min at 37oC in a total reaction volume of 50 μl.

(SKUs: RE001S, RE001L, REc001S, REc001L)

50.00120.00
  • 10u/μl
  • 40-60u/μl
  • 1000 U
  • 3×1000 U

Product Details

AluI is a restriction enzyme purified from Arthrobacter luteus (ATCC 21606)

Reagents supplied: 10x R1 and 10x EQ buffer

Unit definition: One unit is the amount of enzyme required to digest 1 μg of Lambda DNA in 60 min at 37oC in a total reaction volume of 50 μl.

Digest Guidelines

  • Standard reaction conditions: In order to overcome DNA source variability, a ~10 fold overdigest is suggested:
10U AluI1μl
10x EQ buffer5μl
DNA substrate1μg
Sterile ultrapure waterUp to 50 μl
  • Speed Digest reaction: Incubate for 15 minutes at  37oC as in standard reaction in 1x R1 buffer ( 10 mM Tris-HCl (pH 7.9 @ 25oC), 10 mM MgCl2, 100 μg/ml BSA)or 1x EQ buffer (20 mM Tris-acetate (pH 7.9 @ 25oC), 10 mM Mg-acetate, 50 mM K-acetate, 100 μg/ml BSA).
  • Activity in RB buffers-Double digest tips
R1R2R3R4EQ
1001007510-25100

Reactions were performed as in speed digest reaction conditions. The above data could be used as guideline for double digest reactions. Our enzymes are also active and compatible in buffer systems from other vendors.

Heat inactivation: 65oC for 20 minutes.

Methylation Sensitivity:

  • dam methylation: Not sensitive
  • dcm methylation: Not sensitive
  • CpG methylation: Not sensitive

Unit calculation assay conditions:

  • 1x R1 buffer
  • Lambda DNA
  • Incubation at 37oC

Storage conditions:

  • 100 mM KCl,
  • 10 mM Tris-HCl (pH 7.4)
  • 0.1 mM EDTA
  • 1 mM dithiothreitol
  • 200 μg/ml BSA
  • 50% glycerol
  • Store at -20oC

Non-specific nuclease activity: 50 units of AluI do not produce any unspecific cleavage products after 16 hrs incubation with 1 μg of lambda DNA at 37oC.

Re-ligation and recut:After 10-fold overdigestion with Alu I, greater than 95% of the DNA fragments can be ligated and recut with this enzyme.

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