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  Restriction endonucleases

Ava II (isoschizomer)

Ava II is a restriction enzyme purified from an isolated strain.

Reagents supplied: 10x AvaII and 10x EQ buffer

Unit definition: One unit is the amount of enzyme required to digest 1 μg of Lambda DNA in 60 min at 37oC in a total reaction volume of 50 μl.

(SKUs: RE004S, RE004L)

50.00120.00
  • 10u/μl
  • 2000 U
  • 3×2000 U

Product Details

Ava II is a restriction enzyme purified from an isolated strain.

Reagents supplied: 10x AvaII and 10x EQ buffer

Unit definition: One unit is the amount of enzyme required to digest 1 μg of Lambda DNA in 60 min at 37oC in a total reaction volume of 50 μl.

Digest Guidelines

  • Standard reaction conditions: In order to overcome DNA source variability, a ~10 fold overdigest is suggested:
10U Ava II1μl
10x EQ buffer5μl
DNA substrate1μg
Sterile ultrapure waterUp to 50 μl
  • Speed Digest reaction: Incubate for 15 minutes at 37oC as in standard reaction in 1x AvaII buffer (100 mM NaCl, 50 mM Tris-HCl (pH 7.9 @ 25oC), 10 mM MgCl2, 0.02% Triton X-100, 100 μg/ml BSA) or  1x eQ buffer (20 mM Tris-acetate (pH 7.9 @ 25oC), 10 mM Mg-acetate, 50 mM K-acetate, 100 μg/ml BSA).
  • Activity(%) in EnzyQuest buffers -Double digest tips
R1R2R3R4EQ
25-507510050-75100

Reactions were performed as for unit definition reaction. The above data could be used as guideline for double digest reactions. Our enzymes are also active and compatible in buffer systems from other vendors.

Heat inactivation: 65oC for 20 minutes

Methylation Sensitivity:

NOT TESTED

Unit calculation assay conditions:

  • 1x AvaII buffer
  • Lambda DNA
  • Incubation at 37oC

Storage conditions:

  • 50 mM KCl
  • 10 mM Tris-HCl (pH 7.4)
  • 0.1 mM EDTA
  • 1 mM dithiothreitol
  • 200 μg/ml BSA
  • 50% glycerol
  • Store at -20oC

Non-specific nuclease activity: 50 units of iso-Ava II do not produce any unspecific cleavage products after 16 hrs incubation with 1 μg of Lambda DNA at 37oC.

Re-ligation and recut: After 50-fold overdigestion with iso-Ava II, greater than 95% of the DNA fragments can be ligated and recut with this enzyme.

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