EQ SARS-CoV-2 RT-qPCR kit
|RN004S||100 reactions||€385 + VAT|
|RN004M||500 reactions||€1750 + VAT|
|RN004L||>1000 reactions||please call|
For detailed product information please click here.
Product Details (For Research Use Only)
The EQ SARS-CoV-2 RT-qPCR kit is a real-time reverse transcription polymerase chain reaction (rRT-PCR) test. The SARS-CoV-2 primer and probe set is designed according to “WHO interim guidance for laboratory testing for 2019 novel coronavirus (2019-nCoV) in humans”. This kit is based on TaqMan probe real-time fluorescent PCR technology. RNA is extracted and isolated from upper respiratory specimens (nasopharyngeal, oropharyngeal, anterior nasal, and midturbinate swabs, nasopharyngeal wash/aspirates and nasal aspirate specimens). The purified nucleic acid is first reverse-transcribed into cDNA by reverse transcriptase, and then subsequently amplified by Hot start Taq DNA polymerase in the rRT-PCR instrument. In the PCR amplification, the 5′ nuclease activity of Taq DNA polymerase causes the degradation of the TaqMan probe, causing the reporter dye to separate from the quencher dye, generating a fluorescent signal. Fluorescence intensity is monitored at each PCR cycle by the rRT-PCR instrument: FAM and HEX channel for the detection of SARS-CoV-2 E and N gene respectively. Cy5 channel is used for the detection of IPC.
|Buffer 5 X|
|Primer mix 10 X|
|Probe mix A (FAM: E gene, HEX: N gene, Cy5: IPC) 10 X|
|Enzyme Mix 20 X|
Limit of detection
The EQ SARS-CoV-2 RT-qPCR kit can detect up to 4 cps per reaction highlighting its high sensitivity.
QC example: Triplicates of 20 cps and quadruplicates of 4 cps (Exact Diagnostics) per reaction were analyzed. In each reaction 10 ng of total RNA from HeLa cells and 80 ng of carrier RNA are included (Image 1).
Image 1. QC_ protocol. A. The thermocycling program as depicted by CFX manager software, B. Table showing the average Cts and standard deviation obtained after QC run (base line was automatically adjusted by CFX manager program). C. Amplification curves obtained for each transcript. 20 and 4 copies of exact diagnostics template were added in reaction.
Reagent Storage and Handling
· Store the kit below -20°C.
· Freezing and thawing is limited to 5 times.
This product is intended for research use only. The purchaser is responsible to acquire the respective authorization certificates for its particular use