RNA analysis tools

EnzyQuest SARS CoV2 RT qPCR kit_IVD

The EnzyQuest SARS CoV2 RT qPCR kit_IVD is a molecular real time RT-PCR test intended for the qualitative detection of SARS-CoV-2 RNA extracted from human respiratory specimen types collected from patients suspected of COVID-19 by their healthcare provider. High sensitivity down to 5cps of SARS CoV2 RNA per reaction.
RN004S100 reactions€385 + VAT
RN004M500 reactions€1750 + VAT
RN004L>1000 reactionsplease call

For detailed product information please refer to the IFU (ΦΟΧ, ελληνική έκδοση).

Available in two package sizes: RN004S-IVD (96rxns) and RN004M-IVD (480rxns)

Product Details

1. Principle of the Assay 

The SARS-CoV-2 RT-qPCR kit is a real-time reverse transcription polymerase chain reaction (rRT-PCR) test. The SARS-CoV-2 primer and probe set is designed according to “WHO interim guidance for laboratory testing for 2019 novel coronavirus (2019-nCoV) in humans”. This kit is based on TaqMan probe real-time fluorescent PCR technology. Upper respiratory specimens (nasopharyngeal, oropharyngeal, anterior nasal, and midturbinate swabs, nasopharyngeal wash/aspirates and nasal aspirate specimens) are extracted using i.e. QIAamp Viral RNA Mini Kit RNA mini kit (QIAgen). After extraction, the purified nucleic acid is first reverse-transcribed into cDNA by reverse transcriptase, and then subsequently amplified by Hot start Taq DNA polymerase in the rRT-PCR instrument. In the PCR amplification, the 5′ nuclease activity of Taq DNA polymerase causes the degradation of the TaqMan probe, causing the reporter dye to separate from the quencher dye, generating a fluorescent signal. Fluorescence intensity is monitored at each PCR cycle by the rRT-PCR instrument: FAM and HEX channel for the detection of SARS-CoV-2 E and N gene respectively. Cy5 channel is used for the detection of IPC.

2. Kit Contents (Materials Provided) 

Cat NoKit contents Cap
BR033 IVDNuclease Free Waterwhite
BR030IVD5x One Step RT-PCR bufferblue
BR031IVD10x Probe Mix_SARS COV2blue
BR032IVD10x Primer Mix_SARS COV2blue
BR054IVDSARS COV2 Standard (+), 5cp/μlviolet
BR035IVD20x Enzyme Mix One step RT SARS COV2black

3. Reagent Storage and Handling 

  • Kit is shipped on dry ice.
  • All components should immediately be stored between -15°C to -25°C upon arrival.
  • Freezing and thawing is limited to 5 times. 
  • Minimize the temperature difference of the components. 
  • Thaw necessary components just before using and promptly place back in freezer after use. 
  • Protect 10x Probe Mix_SARS COV2 from light during storage and avoid exposure to direct sun light during reaction preparation.
  • See the label for expiration date indicated on packaging.
  • Do not use reagents past their expiration date.


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