window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'UA-169295030-1');

  Restriction endonucleases

Hpa I

HpaI is a restriction enzyme purified from a recombinant E.coli strain.

Reagents supplied: 10x R5 and 10x EQ buffer

Unit definition: One unit is the amount of enzyme required to digest 1 μg of Lambda DNA in 60 min at 37oC in a total reaction volume of 50 μl.

(SKUs: RE018S, RE018L, REc018S, REc018L)

56.00135.00
  • 10u/μl
  • 40-60u/μl
  • 3×800 U
  • 800 U

Product Details

HpaI is a restriction enzyme purified from a recombinant E.coli strain.

Reagents supplied: 10x R5 and 10x EQ buffer

Unit definition: One unit is the amount of enzyme required to digest 1 μg of Lambda DNA in 60 min at 37oC in a total reaction volume of 50 μl.

Digest Guidelines

  • Standard reaction conditions: In order to overcome DNA source variability, a ~10 fold overdigest is suggested:
10U Hpa I1μl
10x EQ buffer5μl
DNA substrate1μg
Sterile ultrapure waterUp to 50 μl
  • Speed Digest reaction: Incubate for 15 minutes at 37oC as in standard reaction in 1x eQ buffer (20 mM Tris-acetate (pH 7.9 @ 25oC), 10 mM Mg-acetate, 50 mM K-acetate, 100 μg/ml BSA).
  • Activity(%) in EnzyQuest buffers -Double digest tips
R1R2R3R4EQ
2525-5025-5010100

Reactions were performed as for unit definition reaction. The above data could be used as guideline for double digest reactions. Our enzymes are also active and compatible in buffer systems from other vendors.

Heat inactivation: No

Methylation Sensitivity:

  • dam methylation: Not sensitive
  • dcm methylation: Not sensitive
  • CpG methylation: Blocked by some combinations of overlapping

Unit calculation assay conditions:

  • 1x EQ buffer
  • Lambda DNA
  • Incubation at 37oC

Storage conditions:

  • 50 mM KCl,
  • 10 mM Tris-HCl (pH 7.4)
  • 0.1 mM EDTA
  • 1 mM dithiothreitol
  • 500 μg/ml BSA
  • 50% glycerol
  • Store at -20oC

Non-specific nuclease activity: 50 units of Hpa I do not produce any unspecific cleavage products after 16 hrs incubation with 1 μg of Lambda DNA at 37oC.

Re-ligation and recut: After 10-fold overdigestion with Hpa I, greater than 95% of the DNA fragments can be ligated and recut with this enzyme.

Star activity: Conditions of high enzyme concentration or glycerol concentration >5%, may result in star activity.

Go to Top