Nucleic Acids & DNA markers

Description

Product Details

The AvaII digest of λ DNA yields the following 36 fragments (in base pairs): 8126, 6555, 6442, 3676, 2606, 2555, 2134, 2005, 1951, 1611*, 1420, 1284, 985, 974, 894, 597, 590, 513, 511, 433, 398, 345, 310, 308, 272, 242, 215, 151, 88, 73, 67, 45, 42, 32, 29* and 23.

*The cohesive ends (12b cos site of bacteriophage λ) of fragments 1611bp and 29 bp may anneal to and form an additional band of 1640 bp. These fragments may be separated by heating to 65^oC for 5 min and then cooling on ice for 3min.

Preparation: Lambda DNA was completely digested by AvaII, phenol extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.

Storage conditions:

• 10 mM Tris-HCl (pH 8.0)
• 1 mM EDTA
• Store at -20^oC

Note: Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH₂O and subsequently heated.