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  Nucleic Acids & DNA markers

Lambda DNA/BstE II Digest Ladder

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The BstE II digest of lambda (λ) DNA yields the following 14 discrete fragments (in base pairs): 8453*, 7242, 6369, 5687*, 4822, 4324, 3675, 2323, 1929, 1371, 1264, 702, 224, 117.   *The cohesive ends (12b cos site of bacteriophage λ) of fragments 8453bp and 5687bp may anneal and form an additional band of 14140bp. These fragments may be separated by heating to 65ºC for 5 min and then cooling on ice for 3min.

(SKU: NM010S, NM010L)

30.00120.00
  • 0.5μg/μl
  • 100μg
  • 500μg

Product Details

The BstE II digest of lambda (λ) DNA yields the following 14 discrete fragments (in base pairs): 8453*, 7242, 6369, 5687*, 4822, 4324, 3675, 2323, 1929, 1371, 1264, 702, 224, 117. 

 *The cohesive ends (12b cos site of bacteriophage λ) of fragments 8453bp and 5687bp may anneal and form an additional band of 14140bp. These fragments may be separated by heating to 65ºC for 5 min and then cooling on ice for 3min. 

Preparation: Lambda DNA was completely digested by BstE II, phenol extracted, ethanol precipitated and dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA. 

Storage conditions:   

  • 10 mM Tris-HCl (pH 8.0)  
  • 1 mM EDTA 
  • Store at -20ºC. 

 Note: Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O and subsequently heated. 

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