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  Nucleic Acids & DNA markers

Lambda DNA/Hind III Digest Ladder

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The Hind III digest of lambda (λ) DNA yields the following 8 discrete fragments (in base pairs): 23130*, 9416, 6557, 4361*, 2322, 2027, 564, 125.  *The cohesive ends (12b cos site of bacteriophage λ) of fragments 23130 and 4361 may be separated by heating to 65ºC for 5 minutes. 

(SKUs: NM005S, NM005L)

30.00120.00
  • 0.5μg/μl
  • 100μg
  • 500μg

Product Details

The Hind III digest of lambda (λ) DNA yields the following 8 discrete fragments (in base pairs): 23130*, 9416, 6557, 4361*, 2322, 2027, 564, 125. 

*The cohesive ends (12b cos site of bacteriophage λ) of fragments 23130 and 4361 may be separated by heating to 65ºC for 5 minutes. 

Preparation: Lambda DNA was completely digested by Hind III, phenol extracted, ethanol precipitated and dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA. 

Storage buffer:   

  • 10 mM Tris-HCl (pH 8.0)  
  • 1 mM EDTA 
  • Store at -20ºC. 

Note: Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O and subsequently heated. 

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