Nucleic Acids & DNA markers

Description

Product Details

The Pst I digest of lambda (λ) DNA yields the following discrete fragments (in base pairs): 11501*, 5077, 4749, 4507, 2838, 2556*, 2459, 2443, 2140, 1986, 1700, 1159 1093, 805, 514, 468, 448, 339, 264, 247, 216, 211, 200, 164, 150, 94, 87, 72, 15. 

 *The cohesive ends (12b cos site of bacteriophage λ) of fragments 11501bp and 2556bp may anneal and form the additional band. These fragments may be separated by heating to 65ºC for 5 min and then cooling on ice for 3min. 

Preparation: Lambda DNA was completely digested by Pst I, phenol extracted, ethanol precipitated and dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA. 

Storage conditions:   

• 10 mM Tris-HCl (pH 8.0)  
• 1 mM EDTA 
• Store at -20ºC. 

 Note: Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O and subsequently heated.