Lambda DNA
Lambda DNA is used as one of the substrates in restriction enzymes research and for testing of restriction endonucleases activity. The molecule is double-stranded DNA, 48,502 base pairs in length, and the molecular weight is 31.5 x 106 daltons.
(SKUs: NM004S, NM004L)
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Product Details
Lambda DNA is used as one of the substrates in restriction enzymes research and for testing of restriction endonucleases activity. The molecule is double-stranded DNA, 48,502 base pairs in length, and the molecular weight is 31.5 x 106 daltons.
Preparation: The molecule is isolated from bacteriophage lambda (cl857 ind1 Sam7). The phage is isolated from the heat inducible lysogen E. coli λ cl857 S7 by gel filtration and the DNA is extracted from the purified phage by phenol/chloroform extraction and dialyzed against 10 mM Tris-HCl (pH 7.4) and 1 mM EDTA.
Quality control: Gel analysis for purity. EcoRI and HindIII fragmentation patterns. More details concerning quality controls could be found in Certificate of analysis.
Storage conditions:
- 10 mM Tris-HCl (pH 7.4)
- 1 mM EDTA
- Store at -20ºC
