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  Nucleic Acids & DNA markers

Lambda DNA

Lambda DNA is used as one of the substrates in restriction enzymes research and for testing of restriction endonucleases activity. The molecule is double-stranded DNA, 48,502 base pairs in length, and the molecular weight is 31.5 x 106 daltons.

(SKUs: NM004S, NM004L)

70.00280.00
  • 0.5μg/μl
  • 0.5mg
  • 2.5mg

Product Details

Lambda DNA is used as one of the substrates in restriction enzymes research and for testing of restriction endonucleases activity. The molecule is double-stranded DNA, 48,502 base pairs in length, and the molecular weight is 31.5 x 106 daltons. 

Preparation: The molecule is isolated from bacteriophage lambda (cl857 ind1 Sam7). The phage is isolated from the heat inducible lysogen E. coli λ cl857 S7 by gel filtration and the DNA is extracted from the purified phage by phenol/chloroform extraction and dialyzed against 10 mM Tris-HCl (pH 7.4) and 1 mM  EDTA. 

Quality control: Gel analysis for purity. EcoRI and HindIII fragmentation patterns. More details concerning quality controls could be found in Certificate of analysis. 

Storage conditions:   

  • 10 mM Tris-HCl (pH 7.4) 
  • 1 mM EDTA 
  • Store at -20ºC 

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