Nucleic Acids & DNA markers
€60.00
pBR322 is a commonly used plasmid cloning vector in E. coli. The molecule is double-stranded circle, 4361 base pairs in length, and has a low copy number. pBR322 carries contains the genes for resistance to ampicillin and tetracycline, and can be amplified with chloramphenicol. The molecular weight of pBR322 is 2.83×106 daltons.
(SKU: NM001)
pBR322 is a commonly used plasmid cloning vector in E. coli. The molecule is double-stranded circle, 4361 base pairs in length, and has a low copy number. pBR322 carries contains the genes for resistance to ampicillin and tetracycline, and can be amplified with chloramphenicol. The molecular weight of pBR322 is 2.83×106 daltons.
Preparation: pBR322 is isolated from E. coli strain HB101 by a standard plasmid purification procedure.
Quality control: Gel analysis for purity. Hinf I and Alu I fragmentation patterns. More details concerning quality controls could be found in Certificate of analysis.
Storage conditions:
Origin of replication: pBM1
Resistant: Ampicillin (Apr), Tetracyclin (TCr)
pBR322 map:
| Concentration | 0.5μg/μl |
|---|---|
| Quantity | 50 μg |
