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  Nucleic Acids & DNA markers

pBR322 DNA plasmid

pBR322 is a commonly used plasmid cloning vector in E. coli. The molecule is double-stranded circle, 4361 base pairs in length, and has a low copy number. pBR322 carries contains the genes for resistance to ampicillin and tetracycline, and can be amplified with chloramphenicol. The molecular weight of  pBR322 is 2.83×106 daltons.

(SKU: NM001)

60.00
  • 0.5μg/μl
  • 50 μg

Product Details

pBR322 is a commonly used plasmid cloning vector in E. coli. The molecule is double-stranded circle, 4361 base pairs in length, and has a low copy number. pBR322 carries contains the genes for resistance to ampicillin and tetracycline, and can be amplified with chloramphenicol. The molecular weight of  pBR322 is 2.83×106 daltons.

Preparation: pBR322 is isolated from E. coli strain HB101 by a standard plasmid purification procedure.

Quality control: Gel analysis for purity. Hinf I and Alu I fragmentation patterns. More details concerning quality controls could be found in Certificate of analysis.

Storage conditions:

  • 10 mM Tris-HCl (pH 8.0)
  • 1 mM EDTA
  • Store at -20oC

Origin of replication: pBM1

Resistant: Ampicillin (Apr), Tetracyclin (TCr)

pBR322 map:

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