Nucleic Acids & DNA markers
€60.00
pUC18 is a commonly used plasmid cloning vector in E. coli. The molecule is double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC18 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases. The molecular weight of pUC18 is 1.75×106 daltons.
(SKU: NM002)
pUC18 is a commonly used plasmid cloning vector in E. coli. The molecule is double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC18 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases. The molecular weight of pUC18 is 1.75×106 daltons.
Preparation: pUC18 is isolated from E. coli strain HB101 by a standard plasmid purification procedure.
Quality control: Gel analysis for purity. BsiS I and Alu I fragmentation patterns. More details concerning quality controls could be found in Certificate of analysis.
Storage conditions:
Origin of replication: pBM1 (mutant)
Resistant: Ampicillin (Apr)
pUC18 map:
Polylinker DNA sequence:
| Concentration | 0.5μg/μl |
|---|---|
| Quantity | 50 μg |
