pUC18 DNA plasmid

pUC18 is a commonly used plasmid cloning vector in E. coli. The molecule is double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC18 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases. The molecular weight of pUC18 is 1.75×106 daltons.

(SKU: NM002)

60.00

Product Details

pUC18 is a commonly used plasmid cloning vector in E. coli. The molecule is double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC18 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases. The molecular weight of pUC18 is 1.75×106 daltons.  

 Preparation: pUC18 is isolated from E. coli strain HB101 by a standard plasmid purification procedure. 

 Quality control: Gel analysis for purity. BsiS I and Alu I fragmentation patterns. More details concerning quality controls could be found in Certificate of analysis. 

 Storage conditions:  

  • 10 mM Tris-HCl (pH 8.0) 
  • 1 mM EDTA 
  • Store at -20ºC 

 Origin of replication: pBM1 (mutant) 

 Resistant: Ampicillin (Apr) 

pUC18 map: 

 Polylinker DNA sequence: 

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