pUC18 DNA plasmid
pUC18 is a commonly used plasmid cloning vector in E. coli. The molecule is double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC18 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases. The molecular weight of pUC18 is 1.75×106 daltons.
(SKU: NM002)
Categories of Products
Other Products in Category
- 100bp DNA Ladder
- 1kbp DNA Ladder
- Lambda DNA
- Lambda DNA EcoR I/Hind III Digest Ladder
- Lambda DNA/Ava II Digest Ladder
- Lambda DNA/BstE II Digest Ladder
- Lambda DNA/EcoR I Digest Ladder
- Lambda DNA/Hind III Digest Ladder
- Lambda DNA/Pst I Digest Ladder
- Lambda DNA/Sty I Digest Ladder
- Long Range DNA Ladder
- pBR322 DNA plasmid
- pBR322 DNA/Hinf I Digest Ladder
- pUC18 DNA plasmid
- pUC19 DNA plasmid
- pUC19 DNA/BseB I/Taq I Digest Ladder
- pUC19 DNA/BsiS I (Hpa II) Digest Ladder
Product Details
pUC18 is a commonly used plasmid cloning vector in E. coli. The molecule is double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC18 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases. The molecular weight of pUC18 is 1.75×106 daltons.
Preparation: pUC18 is isolated from E. coli strain HB101 by a standard plasmid purification procedure.
Quality control: Gel analysis for purity. BsiS I and Alu I fragmentation patterns. More details concerning quality controls could be found in Certificate of analysis.
Storage conditions:
- 10 mM Tris-HCl (pH 8.0)
- 1 mM EDTA
- Store at -20ºC
Origin of replication: pBM1 (mutant)
Resistant: Ampicillin (Apr)
pUC18 map:
Polylinker DNA sequence:
