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  Nucleic Acids & DNA markers

pUC19 DNA plasmid

pUC19 is a commonly used plasmid cloning vector in E. coli. The molecule is double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases. The molecular weight of pUC19 is 1.75×106 daltons 

(SKU: NM003)

60.00
  • 0.5-1μg/μl
  • 50 μg

Product Details

pUC19 is a commonly used plasmid cloning vector in E. coli. The molecule is double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases. The molecular weight of pUC19 is 1.75×106 daltons.  

Preparation: pUC19 is isolated from E. coli strain HB101 by a standard plasmid purification procedure. 

Quality control: Gel analysis for purity. BsiSI and AluI fragmentation patterns. More details concerning quality controls could be found in Certificate of analysis. 

Storage conditions:  

  • 10 mM Tris-HCl (pH 8.0) 
  • 1 mM EDTA 
  • Store at -20ºC

Origin of replication: pBM1 (mutant) 

Resistant: Ampicillin (Apr) 

pUC19 map: 

Polylinker DNA sequence: 

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