pUC19 DNA plasmid
pUC19 is a commonly used plasmid cloning vector in E. coli. The molecule is double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases. The molecular weight of pUC19 is 1.75×106 daltons.
(SKU: NM003)
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Product Details
pUC19 is a commonly used plasmid cloning vector in E. coli. The molecule is double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases. The molecular weight of pUC19 is 1.75×106 daltons.
Preparation: pUC19 is isolated from E. coli strain HB101 by a standard plasmid purification procedure.
Quality control: Gel analysis for purity. BsiSI and AluI fragmentation patterns. More details concerning quality controls could be found in Certificate of analysis.
Storage conditions:
- 10 mM Tris-HCl (pH 8.0)
- 1 mM EDTA
- Store at -20ºC
Origin of replication: pBM1 (mutant)
Resistant: Ampicillin (Apr)
pUC19 map:
Polylinker DNA sequence:
