Sst I (Sac I isoschizomer)
SstI is a restriction enzyme purified from Streptomyces stanford.
Reagents supplied: 10x R1 and 10x EQ buffer
Unit definition: One unit is the amount of enzyme required to digest 1 μg of Lambda DNA (Hind III digest) in 60 min at 37oC in a total reaction volume of 50 μl.
(SKUs: RE041S, RE041L, REc041S, REc041L)
Categories of Products
Other Products in Category
- Alu I
- ApaL I
- Asu II (isoschizomer)
- Ava II (isoschizomer)
- BamH I
- Bcl I
- Bgl I
- Bgl II
- BseB I (BstN I isoschizomer)
- BseC I (Cla I isoschizomer)
- BshF I (Hae III isoschizomer)
- BstE II
- CspA I (Age I isoschizomer)
- EcoR I
- EcoR V
- Hind III
- Hinf I
- Hpa I
- Kpn I
- Mbo I
- MspC I (Afl II isoschizomer)
- Nae I
- Nco I
- Nhe I
- Not I
- Nru I
- PspP I (Sau96 I isoschizomer)
- Pst I
- Pvu II
- Rsa I
- Sal I
- Sau3A I
- Sca I
- Sfi I
- SgrB I (Sac II isoschizomer)
- SlaI (Xho I isoschizomer)
- Sma I
- Sph I
- SseB I (Stu I isoschizomer)
- Ssp I
- Sst I (Sac I isoschizomer)
- Sty I
- Taq I
- XbaI
Product Details
Digest Guidelines
- Standard reaction conditions: In order to overcome DNA source variability, a ~10 fold overdigest is suggested:
| 10U SstI | 1μl |
| 10x EQ buffer | 5μl |
| DNA substrate | 1μg |
| Sterile ultrapure water | Up to 50 μl |
| Incubate for 60 min at 37oC |
- Speed Digest reaction: Incubate for 15 minutes as in standard reaction in 1x R1 or 1x EQ buffer.
- Activity(%) in EnzyQuest buffers -Double digest tips
| R1 | R2 | R3 | R4 | R5 | EQ |
| 100 | 25-50 | 25 | <10 | 50 | – |
Reactions were performed as for unit definition reaction. The above data could be used as guideline for double digest reactions. Our enzymes are also active and compatible in buffer systems from other vendors.
Heat inactivation: 65oC for 20 minutes
Methylation Sensitivity:
dam methylation: Not sensitive
dcm methylation: Not sensitive
CpG methylation: Not sensitive
Unit calculation assay conditions:
- 1x R1 buffer
- Lambda DNA (Hind III digest)
- Incubation at 37ºC
Storage conditions:
- 50 mM NaCl
- 10 mM Tris-HCl (pH 7.4)
- 0.1 mM EDTA
- 1 mM dithiothreitol
- 200 μg/ml BSA
- 50% glycerol
- Store at -20ºC
Non-specific nuclease activity: 100 units of Sst I do not produce any unspecific cleavage products after 16 hrs incubation with 1 μg of Lambda DNA (Hind III digest) at 37oC.
Re-ligation and recut: After 50-fold overdigestion with Sst I, greater than 95% of the DNA fragments can be ligated and recut with this enzyme.
