XbaI
XbaI is a restriction enzyme purified from Xanthomonas badrii.
Reagents supplied: 10x R2 and 10x EQ buffer
Unit definition: One unit is the amount of enzyme required to digest 1 μg of Lambda DNA (dam–/Hind III digest) in 60 min at 37oC in a total reaction volume of 50 μl.
(SKUs: RE044S, RE044L, REc044S, REc044L)
Categories of Products
Other Products in Category
- Alu I
- ApaL I
- Asu II (isoschizomer)
- Ava II (isoschizomer)
- BamH I
- Bcl I
- Bgl I
- Bgl II
- BseB I (BstN I isoschizomer)
- BseC I (Cla I isoschizomer)
- BshF I (Hae III isoschizomer)
- BstE II
- CspA I (Age I isoschizomer)
- EcoR I
- EcoR V
- Hind III
- Hinf I
- Hpa I
- Kpn I
- Mbo I
- MspC I (Afl II isoschizomer)
- Nae I
- Nco I
- Nhe I
- Not I
- Nru I
- PspP I (Sau96 I isoschizomer)
- Pst I
- Pvu II
- Rsa I
- Sal I
- Sau3A I
- Sca I
- Sfi I
- SgrB I (Sac II isoschizomer)
- SlaI (Xho I isoschizomer)
- Sma I
- Sph I
- SseB I (Stu I isoschizomer)
- Ssp I
- Sst I (Sac I isoschizomer)
- Sty I
- Taq I
- XbaI
Product Details
XbaI is a restriction enzyme purified from Xanthomonas badrii.
Reagents supplied: 10x R2 and 10x eQ buffer
Unit definition: One unit is the amount of enzyme required to digest 1 μg of Lambda DNA (dam–/Hind III digest) in 60 min at 37oC in a total reaction volume of 50 μl.
Digest Guidelines
- Standard reaction conditions: In order to overcome DNA source variability, a ~10 fold overdigest is suggested:
10U Xba I | 1μl |
10x eQ buffer | 5μl |
DNA substrate | 1μg |
Sterile ultrapure water | Up to 50 μl |
Incubate for 60 min at 37ºC |
- Speed Digest reaction: Incubate for 15 minutes at 37oC as in standard reaction in 1x R2 buffer (50 mM NaCl, 10 mM Tris-HCl (pH 7.9 @ 25oC), 10 mM MgCl2, 100 μg/ml BSA) or 1x eQ buffer (20 mM Tris-acetate (pH 7.9 @ 25oC), 10 mM Mg-acetate, 50 mM K-acetate, 100 μg/ml BSA).
- Activity(%) in EnzyQuest buffers -Double digest tips
R1 | R2 | R3 | R4 | eQ |
10-25 | 50-75 | 75-100 | 50-75 | 100 |
Reactions were performed as for unit definition reaction. The above data could be used as guideline for double digest reactions. Our enzymes are also active and compatible in buffer systems from other vendors.
Heat inactivation: 65ºC for 20 minutes
Methylation Sensitivity:
- dam methylation: Blocked by overlapping
- dcm methylation: Not sensitive
- CpG methylation: Not sensitive
Unit calculation assay conditions:
- 1x R2 buffer
- Lambda DNA (dam-/Hind III digest)
- Incubation at 37ºC
Storage conditions:
- 100 mM KCl,
- 10 mM Tris-HCl (pH 7.4)
- 0.1 mM EDTA
- 1 mM dithiothreitol
- 500 μg/ml BSA
- 50% glycerol
- Store at -20ºC
Non-specific nuclease activity: 200 units of Xba I do not produce any unspecific cleavage products after 16 hrs incubation with 1 μg of Lambda DNA (dam–/Hind III digest)at 37ºC
Re-ligation and recut: After 100-fold overdigestion with Xba I, greater than 98% of the DNA fragments can be ligated and recut with this enzyme.