EnzyQuest dNTP mix is a mixture of four nucleotides (dATP, dCTP, dGTP, dTTP) in pH 8.5. Each nucleotide is at a concentration of 10 mM. dNTP mix 10mM each is suitable for use in PCR/qPCR, reverse transcription, isothermal amplification, DNA labeling and DNA sequencing.

Form: Clear aqueous solution, pH 8.5

Purity: >99% pure (HPLC tested)

Molecular Weight:

     dATP: 491.18 g/mol (free acid),

     dCTP: 467.15 g/mol (free acid),

     dGTP: 507.18 g/mol (free acid),

     dTTP: 482.17 g/mol (free acid)

Storage: Store at -20^oC

Functional Assays:

PCR: Enzyquest dNTP mix 10 mM each is tested for performance in the polymerase chain reaction (PCR) using 1.25 units of Taq DNA polymerase to amplify a 5000-bp region of Lambda DNA using 20ng as substrate and a final concentration 0.2mM each dNTP, in 50 μl total reaction volume. The resulting PCR product is visualized as a single band on an midori green-stained agarose gel.

RT-PCR: Enzyquest dNTP mix 10 mM each is tested for performance in a first strand cDNA synthesis using 1μl dNTP mix and 500ng RNA as a substrate following by a PCR reaction as described on functional assay, PCR. The resulting 1600 bp PCR product is visualized as a single band on an midori green-stained agarose gel.

Quality controls:

Tested extensively for the absence of endo- and exodeoxyribonucleases. More details concerning quality controls could be found in Certificate of analysis.

Stability:

dNTP mix 10mM each is guaranteed to maintain stability for twelve months from the date of shipment when stored as directed.